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pAG416-Gal-PR50
(Plasmid #84901)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84901 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG416-Gal-ccdB
  • Backbone manufacturer
    Susan Lindquist (Addgene plasmid # 14147)
  • Backbone size w/o insert (bp) 5647
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PR50
  • Alt name
    C9orf72 dipeptide
  • Species
    Synthetic
  • Insert Size (bp)
    378
  • Entrez Gene
    C9orf72 (a.k.a. ALSFTD, DENND9, DENNL72, FTDALS, FTDALS1)
  • Promoter GAL1
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG416-Gal-PR50 was a gift from Aaron Gitler (Addgene plasmid # 84901 ; http://n2t.net/addgene:84901 ; RRID:Addgene_84901)

  • For your References section:

    Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS. Jovicic A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada SB, Paul JW 3rd, Sun S, Herdy JR, Bieri G, Kramer NJ, Gage FH, Van Den Bosch L, Robberecht W, Gitler AD. Nat Neurosci. 2015 Sep;18(9):1226-9. doi: 10.1038/nn.4085. 10.1038/nn.4085 PubMed 26308983
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