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Addgene

HOXA4 (murine) HA-tag pET
(Plasmid #8489)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA4
  • Alt name
    HOX1D
  • Alt name
    HOX1.4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    856
  • Mutation
    Thr-2 changed to Ala-2 to form better ribosomal binding site
  • Entrez Gene
    Hoxa4 (a.k.a. AV206827, Hox-1.4, MGC130055)
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI/XhoI blunt (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Deborah Wolgemuth

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

improved ribosomal binding site:
TTC ACC ATG G

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA4 (murine) HA-tag pET was a gift from Corey Largman (Addgene plasmid # 8489 ; http://n2t.net/addgene:8489 ; RRID:Addgene_8489)

  • For your References section:

    Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, Largman C. Nucleic Acids Res 1996 Mar 1;24(5):898-906. 10.1093/nar/24.5.898 PubMed 8600458
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