pAAV-Sico-Red
(Plasmid #84882)

• Purpose: Cre-inducible shRNA
• Depositing Lab: Eun Mi Hwang
• Publication: Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi: 10.5607/en.2016.25.3.120. Epub 2016 Jun 22.

Backbone

• Vector backbone: pAAV-MCS
• Backbone manufacturer: stratagene
• Backbone size w/o insert (bp): 3417
• Total vector size (bp): 7479
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sico-Red
• Insert Size (bp): 4062
• Promoter: EF1a and CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (unknown if destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: CMV-pro
• 3′ sequencing primer: bGH-pA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid was modifed from pSico(addgene #11578) vector.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS identified that this plasmid includes the Best1 targeted sequence downstream of GFP. See the associated publication (PMID: 27358580) for additional information.