2 gRNA concatmer
(Plasmid
#84879)
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Purpose(Empty Backbone) Empty concatmer vector in which 2 gRNAs can be inserted
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCVpuro
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Backbone manufacturerClontech
- Backbone size (bp) 6500
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2 gRNA concatmer was a gift from Bon-Kyoung Koo (Addgene plasmid # 84879 ; http://n2t.net/addgene:84879 ; RRID:Addgene_84879) -
For your References section:
Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Andersson-Rolf A, Merenda A, Mustata RC, Li T, Dietmann S, Koo BK. Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 10.1016/j.ydbio.2016.10.016. 10.1016/j.ydbio.2016.10.016 PubMed 27983963