pET15b LFn-Fla 3A
(Plasmid #84872)

• Purpose: Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
• Depositing Lab: Russell Vance
• Publication: Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub 2016 Apr 4.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: LFn
• Alt name: N-terminal domain of anthrax lethal factor
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: Fla
• Alt name: flagellin
• Alt name: FlaA
• Species: L. pneumophila
• Mutation: 3 C-terminal leucines have been replaced by alanine
• GenBank ID: YP_095369

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: T7 terminal

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry