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Addgene

pEMdelta
(Plasmid #8487)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrc99A
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 4163
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miniTN7
  • Alt name
    tnsA
  • Species
    Bacteria
  • Insert Size (bp)
    1763
  • Entrez Gene
    tnsA (a.k.a. R721_29)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI? (not destroyed)
  • 3′ cloning site BamHI? (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMdelta was a gift from Nancy Craig (Addgene plasmid # 8487 ; http://n2t.net/addgene:8487 ; RRID:Addgene_8487)

  • For your References section:

    Tn7 transposition: target DNA recognition is mediated by multiple Tn7-encoded proteins in a purified in vitro system. Bainton RJ, Kubo KM, Feng JN, Craig NL. Cell 1993 Mar 26;72(6):931-43. 10.1016/0092-8674(93)90581-A PubMed 8384534
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