pFastBacDual with LFn + Needle
(Plasmid
#84868)
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PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
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Depositing Lab
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Sequence Information
Ordering
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| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBacDual
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Backbone manufacturerThermoFisher
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Vector typeInsect Expression ; Baculovirus
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameLFn
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Alt nameN-terminal domain of anthrax lethal factor
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameNeedle
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Alt nameBTH_RS04290
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Alt nameT3SS needle protein
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SpeciesB. thailandensis
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GenBank IDWP_009896110
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBacDual with LFn + Needle was a gift from Russell Vance (Addgene plasmid # 84868 ; http://n2t.net/addgene:84868 ; RRID:Addgene_84868) -
For your References section:
NAIP proteins are required for cytosolic detection of specific bacterial ligands in vivo. Rauch I, Tenthorey JL, Nichols RD, Al Moussawi K, Kang JJ, Kang C, Kazmierczak BI, Vance RE. J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub 2016 Apr 4. 10.1084/jem.20151809 PubMed 27045008
