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Addgene

pRM2
(Plasmid #8486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 2684
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    attTn7
  • Alt name
    Tn7 target site
  • Alt name
    tnsA
  • Species
    Bacteria
  • Insert Size (bp)
    505
  • Entrez Gene
    tnsA (a.k.a. R721_29)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AccI (destroyed during cloning)
  • 3′ cloning site AccI (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRM2 was a gift from Nancy Craig (Addgene plasmid # 8486 ; http://n2t.net/addgene:8486 ; RRID:Addgene_8486)

  • For your References section:

    Sequence requirements of Escherichia coli attTn7, a specific site of transposon Tn7 insertion. McKown RL, Orle KA, Chen T, Craig NL. J Bacteriol 1988 Jan;170(1):352-8. PubMed 2826397
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