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Addgene

pCW15
(Plasmid #8485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC184
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 4244
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tn7ABC
  • Alt name
    tnsA
  • Alt name
    tnsB
  • Alt name
    tnsC
  • Species
    Bacteria
  • Insert Size (bp)
    4923
  • Entrez Gene
    tnsA (a.k.a. R721_29)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW15 was a gift from Nancy Craig (Addgene plasmid # 8485 ; http://n2t.net/addgene:8485 ; RRID:Addgene_8485)

  • For your References section:

    Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes. Waddell CS, Craig NL. Genes Dev 1988 Feb;2(2):137-49. 10.1101/gad.2.2.137 PubMed 2834269
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