pCW4
(Plasmid #8484)

• Depositing Lab: Nancy Craig
• Publication: Waddell et al Genes Dev 1988 Feb;2(2):137-49.

Backbone

• Vector backbone: pACYC184
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 4245
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tn7ABCDE
• Alt name: tnsA
• Alt name: tnsB tnsC
• Alt name: tnsD tnsE
• Species: Bacteria
• Insert Size (bp): 8552
• Entrez Gene: tnsA (a.k.a. R721_29)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCW4 sequence is not complete: the entire plasmid is not known (provided sequence is simply a mosaic of the known pieces). Note: Sequence around 8554 RI site uncertain, there is about 800 bp extra between the first RI site and the adjacent HIII site.