pBabe-HA-PHLPP1-delta-PDZ
(Plasmid #84825)

• Purpose: Mammalian expression of a HA tagged, human PHLPP1 containing a deletion of the PDZ binding motif
• Depositing Lab: Alexandra Newton
• Publication: Reyes et al Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):E3957-65. doi: 10.1073/pnas.1404221111. Epub 2014 Sep 8.

Backbone

• Vector backbone: pBabe puro
• Backbone size w/o insert (bp): 5176
• Total vector size (bp): 10322
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phlpp1
• Alt name: SCOP
• Species: H. sapiens (human)
• Mutation: Deletion of amino acids 1714-1717
• GenBank ID: NM_194449
• Entrez Gene: PHLPP1 (a.k.a. PHLPP, PLEKHE1, PPM3A, SCOP)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBabe 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry