myc mTOR kinase dead
(Plasmid #8482)

• Depositing Lab: David Sabatini
• Publication: myc mTOR kinase dead (unpublished)

Backbone

• Vector backbone: prk5
• Backbone size w/o insert (bp): 4716
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTOR
• Species: R. norvegicus (rat)
• Insert Size (bp): 4500
• Mutation: D2357E V2364I
• Entrez Gene: Mtor (a.k.a. Frap1, RAFT1)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: sal (unknown if destroyed)
• 3′ cloning site: not (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: we cloned this gene
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NOTE: Though the depositors list SalI and NotI as the 5' and 3' cloning sites, respectively, Addgene's quality control sequence shows that there is actually only a SalI at the 3' end of the insert. There is no NotI present, so it may have been destroyed. Please refer to Addgene's sequence for further sub-cloning options.