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Addgene

myc GbetaL mutant 5
(Plasmid #8479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    prk5
  • Backbone size w/o insert (bp) 4716
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GbetaL
  • Alt name
    G protein beta subunit-like
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • Mutation
    G192D
  • Entrez Gene
    MLST8 (a.k.a. GBL, GbetaL, LST8, POP3, WAT1)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sal (unknown if destroyed)
  • 3′ cloning site not (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    we cloned this gene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myc GbetaL mutant 5 was a gift from David Sabatini (Addgene plasmid # 8479 ; http://n2t.net/addgene:8479 ; RRID:Addgene_8479)

  • For your References section:

    GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim DH, Sarbassov DD, Ali SM, Latek RR, Guntur KV, Erdjument-Bromage H, Tempst P, Sabatini DM. Mol Cell 2003 Apr;11(4):895-904. 10.1016/S1097-2765(03)00114-X PubMed 12718876
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