pET - FnR062.Y78F
(Plasmid
#84788)
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Purposebacterial expression of FnR.0.6.2-Y78F protein
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)T7 express
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFnR.0.6.2-Y78F
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SpeciesH. sapiens (human)
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MutationY78F
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET - FnR062.Y78F was a gift from Benjamin Hackel (Addgene plasmid # 84788 ; http://n2t.net/addgene:84788 ; RRID:Addgene_84788) -
For your References section:
Development and Implementation of a Protein–Protein Binding Experiment To Teach Intermolecular Interactions in High School or Undergraduate Classrooms. Johnson SM, Javner C, Hackel BJ. J. Chem. Educ. 2017, 94, 3, 367–374 10.1021/acs.jchemed.6b00323