pEX-A-U6-MaSgRNA_PuroR
(Plasmid #84780)

• Purpose: Expresses major satellite-specific sgRNA.
• Depositing Lab: Heinrich Leonhardt
• Publication: Schmidtmann et al Nucleus. 2016 Sep 27:0.

Backbone

• Vector backbone: pEX-A
• Backbone size w/o insert (bp): 5314
• Total vector size (bp): 5334
• Vector type: Mammalian Expression, Bacterial Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: major satellite-specific sgRNA
• gRNA/shRNA sequence: GGCAAGAAAACTGAAAATCA
• Promoter: U6

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5´- GAG GGC CTA TTT CCC ATG ATT C-3´

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry