pCAG-BirA*-dCas9-eGFP
(Plasmid #84779)

• Purpose: Expresses BirA*-dCas9-eGFP in mammalian cells.
• Depositing Lab: Heinrich Leonhardt
• Publication: Schmidtmann et al Nucleus. 2016 Sep 27:0.

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 6127
• Total vector size (bp): 12190
• Vector type: Mammalian Expression, Bacterial Expression, CRISPR
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BirA*-dCas9-eGFP
• Insert Size (bp): 6063
• Promoter: CAG
• Tag / Fusion Protein:
  • cMyc (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: 5´-CAGGGCGGGGTTCGGC-3'
• 3′ sequencing primer: 5´-CCAAGCGGCTTCGGCAG-3´

Resource Information

• A portion of this plasmid was derived from a plasmid made by: hCas9_D10A was received via addgene (addgene ID: 41816), 2nd mutation (H840A) was introduced in our lab. BirA* was received via addgene (addgene ID: 35700)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry