BTV
(Plasmid
#84771)
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Purpose(Empty Backbone) bidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein production
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBdLV
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Backbone manufacturerLuigi Naldini , PMID 15619618
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Modifications to backboneThe constitutive bidirectional promoter in BdLV was replaced with a bidirectional tetracycline responsive promoter. A linker with MluI, SbfI and PacI sites was inserted between the EGFP open reading frame and the polyA signal for subcloning of 3' UTR sequences
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BTV was a gift from David Erle (Addgene plasmid # 84771 ; http://n2t.net/addgene:84771 ; RRID:Addgene_84771) -
For your References section:
Massively parallel functional annotation of 3' untranslated regions. Zhao W, Pollack JL, Blagev DP, Zaitlen N, McManus MT, Erle DJ. Nat Biotechnol. 2014 Apr;32(4):387-91. doi: 10.1038/nbt.2851. Epub 2014 Mar 16. 10.1038/nbt.2851 PubMed 24633241
Map uploaded by the depositor.
