myc GbetaL mutant 1
(Plasmid #8477)

• Depositing Lab: David Sabatini
• Publication: Kim et al Mol Cell 2003 Apr;11(4):895-904.

Backbone

• Vector backbone: prk5
• Backbone size w/o insert (bp): 4716
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GbetaL
• Alt name: G protein beta subunit-like
• Species: H. sapiens (human)
• Insert Size (bp): 1080
• Mutation: D42A
• Entrez Gene: MLST8 (a.k.a. GBL, GbetaL, LST8, POP3, WAT1)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: sal (unknown if destroyed)
• 3′ cloning site: not (unknown if destroyed)
• 5′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: we cloned this gene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry