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PurposeLenti virus delivery of LbCpf1 and crRNA guide
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 84740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHKO_23
- Backbone size w/o insert (bp) 8069
- Total vector size (bp) 12630
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namehuLbCpf1
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Alt namehuLbCas12a
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SpeciesSynthetic
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Insert Size (bp)3867
- Promoter EFS
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Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS (C terminal on insert)
- 3xHA (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer aggtcttgaaaggagtgggaattgg (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namepuromycin resistance gene
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Alt namepuro
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SpeciesSynthetic
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Insert Size (bp)597
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Tag
/ Fusion Protein
- P2A (N terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains a BsmBI cloning cassette for insertion of Cpf1 guides under U6 promoter control.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pY109 (lenti-LbCpf1) was a gift from Feng Zhang (Addgene plasmid # 84740 ; http://n2t.net/addgene:84740 ; RRID:Addgene_84740) -
For your References section:
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. 10.1038/nbt.3737 PubMed 27918548