HA Raptor mutant 4
(Plasmid
#8474)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneprk5
- Backbone size w/o insert (bp) 4716
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRaptor
-
SpeciesH. sapiens (human)
-
Mutation391SQ392-PA (S391P, Q392A)
-
Entrez GeneRPTOR (a.k.a. KOG1, Mip1)
-
Tag
/ Fusion Protein
- ha (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site sal (unknown if destroyed)
- 3′ cloning site not (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bywe cloned this gene
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HA Raptor mutant 4 was a gift from David Sabatini (Addgene plasmid # 8474 ; http://n2t.net/addgene:8474 ; RRID:Addgene_8474) -
For your References section:
mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Cell 2002 Jul 26;110(2):163-75. 10.1016/S0092-8674(02)00808-5 PubMed 12150925