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Addgene

pGem-nHyPer:sAPX Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extend ladder and a digestion of the plasmid pGem-nHyPer:sAPX with the following enzymes: SnaBI and NheI-HF.

We predict the following pattern:

SnaBI - 19.3 kb
NheI-HF - 19.3 kb
SnaBI + NheI-HF - 13.2kb & 6.1kb

We see the expected result.