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pVR2-m1
(Plasmid #84720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 2882
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    E. coli RecA promoter
  • Species
    E. coli
  • Insert Size (bp)
    42
  • GenBank ID
    NC_000913.3 (2,823,819..2,823,860) NC_000913.3

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    stretch lacking A's followed by 5' UTR of queC gene
  • Alt name
    preQ1 riboswitch
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    163
  • Mutation
    C to U mutation at position 13 of annotated "BSU_misc_RNA_22"
  • GenBank ID
    NC_000964.3 (1,439,265..1,439,415) NC_000964.3

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Catherine Eichhorn at the University of Michigan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVR2-m1 was a gift from Nils Walter (Addgene plasmid # 84720 ; http://n2t.net/addgene:84720 ; RRID:Addgene_84720)

  • For your References section:

    Ligand Modulates Cross-Coupling between Riboswitch Folding and Transcriptional Pausing. Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch I, Walter NG. Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 10.1016/j.molcel.2018.08.046. 10.1016/j.molcel.2018.08.046 PubMed 30388413
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