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Addgene

HA Raptor mutant 2
(Plasmid #8471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    prk5
  • Backbone size w/o insert (bp) 4716
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Raptor
  • Species
    H. sapiens (human)
  • Mutation
    261DLF263-AAA (D261A, L262A, F263A)
  • Entrez Gene
    RPTOR (a.k.a. KOG1, Mip1)
  • Tag / Fusion Protein
    • ha (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sal (unknown if destroyed)
  • 3′ cloning site not (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    we cloned this gene
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA Raptor mutant 2 was a gift from David Sabatini (Addgene plasmid # 8471 ; http://n2t.net/addgene:8471 ; RRID:Addgene_8471)

  • For your References section:

    mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Cell 2002 Jul 26;110(2):163-75. 10.1016/S0092-8674(02)00808-5 PubMed 12150925
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