HA Raptor mutant 2
(Plasmid #8471)

• Depositing Lab: David Sabatini
• Publication: Kim et al Cell 2002 Jul 26;110(2):163-75.

Backbone

• Vector backbone: prk5
• Backbone size w/o insert (bp): 4716
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Raptor
• Species: H. sapiens (human)
• Mutation: 261DLF263-AAA (D261A, L262A, F263A)
• Entrez Gene: RPTOR (a.k.a. KOG1, Mip1)
• Tag / Fusion Protein:
  • ha (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: sal (unknown if destroyed)
• 3′ cloning site: not (unknown if destroyed)
• 5′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: we cloned this gene
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry