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Addgene

Cathepsin S S21C/C25A
(Plasmid #84695)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAPSPEGFP1.4sat
  • Backbone manufacturer
    Jena Bioscience
  • Backbone size w/o insert (bp) 7655
  • Vector type
    expression in Leishmania tarantolae
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cathepsin S
  • Alt name
    antigenprocessing proteases
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    945
  • Mutation
    S21C, C25A
  • Entrez Gene
    CTSS
  • Promoter unknown
  • Tag / Fusion Protein
    • his-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

expression constructs contain an N-terminal signal sequence for secretory expression, followed by an N-terminal His6-tag for purification, which remains with the propeptide after autoactivation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cathepsin S S21C/C25A was a gift from Hans Brandstetter (Addgene plasmid # 84695 ; http://n2t.net/addgene:84695 ; RRID:Addgene_84695)

  • For your References section:

    Protease recognition sites in Bet v 1a are cryptic, explaining its slow processing relevant to its allergenicity. Freier R, Dall E, Brandstetter H. Sci Rep. 2015 Aug 3;5:12707. doi: 10.1038/srep12707. 10.1038/srep12707 PubMed 26235974
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