pCis mCherry-SS Himar1 A7
(Plasmid #84687)

• Purpose: (Empty Backbone) Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.
• Depositing Lab: Ulrike Munderloh
• Publication: Munderloh et al Intracellular Pathogens II: Rickettsiales. Vol. 89, No. 3, 2012. Chapter 14. eds: Palmer GH, and Azad AF. ASM Press, New York.

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression
• Selectable markers: Spectinomycin and Streptomycin, mCherry

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry