pCis GFPuv-SS Himar1 A7
(Plasmid #84686)

• Purpose: (Empty Backbone) Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.
• Depositing Lab: Ulrike Munderloh
• Publication: Munderloh et al Intracellular Pathogens II: Rickettsiales. Vol. 89, No. 3, 2012. Chapter 14. eds: Palmer GH, and Azad AF. ASM Press, New York.

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression
• Selectable markers: Spectinomycin and Streptomycin, GFPuv

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Based on pMarGent, received from the RML (http://aem.asm.org/content/70/10/5973.full) The flgB promoter was replaced with an optimized Anaplasma marginale tr promoter. Initially, we used a two-plasmid system with the transposase and transposon encoded on two separate plasmids, described here: http://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-6-42 . pCis GFPuv-SS Himar1 A7 contains transposase and transposon in one plasmid and is described in Chapter 14 of Intracellular Pathogens II: Rickettsiales, Edited by G. H. Palmer and A. F. Azad © 2012 ASM Press, Washington, DC, 10.1128/9781555817336.ch14