pRAM18dSFA[MCS]
(Plasmid
#84685)
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Purpose(Empty Backbone) Can be used for the transformation of rickettsia
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-3Z
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Backbone manufacturerPromega
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Vector typeBacterial Expression
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Selectable markersSpectinomycin and Streptomycin, mKate
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRAM18dSFA[MCS] was a gift from Ulrike Munderloh (Addgene plasmid # 84685 ; http://n2t.net/addgene:84685 ; RRID:Addgene_84685) -
For your References section:
Development of shuttle vectors for transformation of diverse Rickettsia species. Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, Felsheim RF, Kurtti TJ, Munderloh UG. PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub 2011 Dec 21. 10.1371/journal.pone.0029511 PubMed 22216299
Map uploaded by the depositor.
