HA GST F5A deltaCT S6K1 pRK5
(Plasmid
#8467)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneprk5
- Backbone size w/o insert (bp) 4716
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namef5a delta CT p85 s6k1
-
Alt namep70 s6k1 alpha I
-
Alt nameRps6kb1
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)1200
-
Entrez GeneRps6kb1 (a.k.a. p70 S6K-alpha)
-
Tag
/ Fusion Protein
- ha/gst (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site sal (not destroyed)
- 3′ cloning site not (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ha/gst prk5 is homegrown vector made by David M. Sabatini
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HA GST F5A deltaCT S6K1 pRK5 was a gift from David Sabatini (Addgene plasmid # 8467 ; http://n2t.net/addgene:8467 ; RRID:Addgene_8467) -
For your References section:
Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali SM, Sabatini DM. J Biol Chem 2005 Apr 4;. 10.1074/jbc.C500125200 PubMed 15809305