HA GST p85 S6K1 pRK5
(Plasmid #8466)

• Depositing Lab: David Sabatini
• Publication: Ali et al J Biol Chem 2005 Apr 4;.

Backbone

• Vector backbone: prk5
• Backbone size w/o insert (bp): 4716
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p85 s6k1
• Alt name: s6k1 alpha I
• Alt name: Rps6kb1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1647
• Entrez Gene: Rps6kb1 (a.k.a. p70 S6K-alpha)
• Tag / Fusion Protein:
  • ha/gst (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: sal (not destroyed)
• 3′ cloning site: not (not destroyed)
• 5′ sequencing primer: pGEX5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry