Skip to main content
Addgene

pLCNICK
(Plasmid #84653)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCas
  • Backbone manufacturer
    Professor Sheng Yang (Plasmid 62225)
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 14300
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9 nickase
  • Species
    S. pyogenes
  • Insert Size (bp)
    4107
  • Mutation
    D10A
  • GenBank ID
    AE004092

Gene/Insert 2

  • Gene/Insert name
    sgRNA
  • Insert Size (bp)
    103

Gene/Insert 3

  • Gene/Insert name
    homology arms of LC2W_2179
  • Insert Size (bp)
    2007

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCNICK was a gift from Sheng Yang (Addgene plasmid # 84653 ; http://n2t.net/addgene:84653 ; RRID:Addgene_84653)

  • For your References section:

    CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Song X, Huang H, Xiong Z, Ai L, Yang S. Appl Environ Microbiol. 2017 Sep 1. pii: AEM.01259-17. doi: 10.1128/AEM.01259-17. 10.1128/AEM.01259-17 PubMed 28864652
Related items:
Commonly requested with: