pRARE-MBP-DEST
(Plasmid
#84650)
-
Purpose(Empty Backbone) Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepRARE
-
Backbone manufacturerEMD Chemicals
-
Modifications to backbonemalE expression cassette of pMAL-c2 (New England Biolabs) was modified for Gateway cloning. The region containing the lacIq gene and modified malE expression cassette was then subcloned into the pRARE plasmid.
-
Vector typeBacterial Expression
- Promoter tac
-
Tag
/ Fusion Protein
- MBP (N terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DB3.1
-
Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MBP-F
- 3′ sequencing primer pBAD-R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRARE-MBP-DEST was a gift from Neil Olszewski (Addgene plasmid # 84650 ; http://n2t.net/addgene:84650 ; RRID:Addgene_84650) -
For your References section:
The Arabidopsis O-linked N-acetylglucosamine transferase SPINDLY interacts with class I TCPs to facilitate cytokinin responses in leaves and flowers. Steiner E, Efroni I, Gopalraj M, Saathoff K, Tseng TS, Kieffer M, Eshed Y, Olszewski N, Weiss D. Plant Cell. 2012 Jan;24(1):96-108. doi: 10.1105/tpc.111.093518. Epub 2012 Jan 20. 10.1105/tpc.111.093518 PubMed 22267487
