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pCuo DN Rac1
(Plasmid #84644)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    QM516B-1
  • Backbone manufacturer
    System Biosciences
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin ; RFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rac1
  • Species
    H. sapiens (human)
  • Mutation
    Dominant Negative
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
  • Promoter cumate-inducible promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CACCTGGCCCGATCTGGCC (from SparQ Cumate Switch System Biosciences manual)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCuo DN Rac1 was a gift from Sanjay Kumar (Addgene plasmid # 84644 ; http://n2t.net/addgene:84644 ; RRID:Addgene_84644)

  • For your References section:

    Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters. MacKay JL, Kumar S. Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d. 10.1039/c4ib00099d PubMed 25044255
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