pcDNA3-mCherry-LKB1(KD)
(Plasmid #84640)

• Purpose: mCherry-tagged, LKB1(K78I); kinase-dead mutant
• Depositing Lab: Jin Zhang
• Publication: Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. doi: 10.1016/j.chembiol.2015.10.004. Epub 2015 Nov 5.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7509
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-LBK1(K78I)
• Alt name: LKB1 (KD)
• Alt name: STK11
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2022
• Mutation: Lysine 78 mutated to Isoleucine; kinase dead
• GenBank ID: NC_000019.10
• Entrez Gene: STK11 (a.k.a. LKB1, PJS, hLKB1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • 6xHis (N terminal on backbone)
  • T7 tag (gene 10 leader) (N terminal on backbone)
  • Xpress (TM) tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-mCherry-LKB1(KD).gb
• A portion of this plasmid was derived from a plasmid made by: Lewis Cantley (Addgene Plasmid #8591)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry