pHR_MFE1_hrGFP
(Plasmid #84617)

• Purpose: HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus
• Depositing Lab: Ian Wheeldon
• Publication: Schwartz et al ACS Synth Biol. 2016 Dec 19.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2623
• Total vector size (bp): 8873
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hrGFP
• Insert Size (bp): 720
• Promoter: UAS1B8-TEF(136)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BssHII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: TEF
• 3′ sequencing primer: CYC

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Addgene material #44380 (UAS1B8-TEF promoter) is used to express hrGFP
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry