pCRISPRyl_AXP
(Plasmid #84608)

• Purpose: Introduces DSB at AXP locus in Y. lipolytica
• Depositing Lab: Ian Wheeldon
• Publication: Schwartz et al ACS Synth Biol. 2016 Dec 19.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2623
• Total vector size (bp): 11822
• Vector type: Yeast Expression, CRISPR, Synthetic Biology
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• gRNA/shRNA sequence: GACCAGGTCGAAGTAGCTGG
• Promoter: UAS1B8-TEF(136)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BssHII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: TEF
• 3′ sequencing primer: CYC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene material #44380 (UAS1B8-TEF promoter) is used to express Cas9
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry