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Addgene

pGEX-HCF-1rep1wt
(Plasmid #84606)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84606 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-6P-2
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5615
  • Modifications to backbone
    Addition of TEV protease site at 5' end of cloning site and 6x His tag at 3' end of cloning site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCF-1rep1wt
  • Alt name
    HCFC1rep1wt
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    615
  • Entrez Gene
    HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
  • Promoter T7
  • Tags / Fusion Proteins
    • GST-TEV (N terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GEX5-for
  • 3′ sequencing primer GEX3-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-HCF-1rep1wt was a gift from Winship Herr (Addgene plasmid # 84606 ; http://n2t.net/addgene:84606 ; RRID:Addgene_84606)
  • For your References section:

    O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1. Capotosti F, Guernier S, Lammers F, Waridel P, Cai Y, Jin J, Conaway JW, Conaway RC, Herr W. Cell. 2011 Feb 4;144(3):376-88. doi: 10.1016/j.cell.2010.12.030. 10.1016/j.cell.2010.12.030 PubMed 21295698