pGEX-HCF-1rep1wt
(Plasmid #84606)

• Purpose: Plasmid contains HCF-1rep1wt with GST-tag
• Depositing Lab: Winship Herr
• Publication: Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.1016/j.cell.2010.12.030.

Backbone

• Vector backbone: pGEX-6P-2
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5615
• Modifications to backbone: Addition of TEV protease site at 5' end of cloning site and 6x His tag at 3' end of cloning site
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HCF-1rep1wt
• Alt name: HCFC1rep1wt
• Species: H. sapiens (human)
• Insert Size (bp): 615
• Entrez Gene: HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
• Promoter: T7
• Tags / Fusion Proteins:
  • GST-TEV (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GEX5-for
• 3′ sequencing primer: GEX3-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry