pGEX-HCF-1rep1wt
(Plasmid
#84606)
-
PurposePlasmid contains HCF-1rep1wt with GST-tag
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 84606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGEX-6P-2
-
Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5615
-
Modifications to backboneAddition of TEV protease site at 5' end of cloning site and 6x His tag at 3' end of cloning site
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHCF-1rep1wt
-
Alt nameHCFC1rep1wt
-
SpeciesH. sapiens (human)
-
Insert Size (bp)615
-
Entrez GeneHCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
- Promoter T7
-
Tags
/ Fusion Proteins
- GST-TEV (N terminal on backbone)
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GEX5-for
- 3′ sequencing primer GEX3-rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEX-HCF-1rep1wt was a gift from Winship Herr (Addgene plasmid # 84606 ; http://n2t.net/addgene:84606 ; RRID:Addgene_84606) -
For your References section:
O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1. Capotosti F, Guernier S, Lammers F, Waridel P, Cai Y, Jin J, Conaway JW, Conaway RC, Herr W. Cell. 2011 Feb 4;144(3):376-88. doi: 10.1016/j.cell.2010.12.030. 10.1016/j.cell.2010.12.030 PubMed 21295698