pGL3b-8XGliBS:EGFP
(Plasmid #84602)

• Purpose: Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling
• Depositing Lab: James Chen
• Publication: Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14132-7. doi: 10.1073/pnas.0907134106. Epub 2009 Aug 5.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Species: jellyfish
• Insert Size (bp): 714
• Promoter: 8X Gli-binding site

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: EBV-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid derived from pGl3-B-8XGliBS:Luc (Sasaki, et al., Development, 1997)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid generated by replacing the firefly luciferase cDNA in the 8XGliBS:Luc vector (Sasaki, et al, 1997) with EGFP cDNA from the pEGFP-C1 vector (Clontech).