pCIneoRL-KLF9 3'UTR
(Plasmid
#84599)
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PurposeRenilla luciferase reporter containing KLF9 3'UTR
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCIneoRL
- Backbone size w/o insert (bp) 6380
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKLF9 3'UTR
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SpeciesH. sapiens (human)
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Insert Size (bp)965
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Entrez GeneKLF9 (a.k.a. BTEB, BTEB1)
- Promoter CMV
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Tag
/ Fusion Protein
- Renilla Luciferase (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer RenLuc3F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIneoRL-KLF9 3'UTR was a gift from Yanan Yang (Addgene plasmid # 84599 ; http://n2t.net/addgene:84599 ; RRID:Addgene_84599) -
For your References section:
A genetic cell context-dependent role for ZEB1 in lung cancer. Zhang T, Guo L, Creighton CJ, Lu Q, Gibbons DL, Yi ES, Deng B, Molina JR, Sun Z, Yang P, Yang Y. Nat Commun. 2016 Jul 26;7:12231. doi: 10.1038/ncomms12231. 10.1038/ncomms12231 PubMed 27456471