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pCIneoRL-Notch1 3'UTR-mir200c-mut
(Plasmid #84596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIneoRL
  • Backbone size w/o insert (bp) 6380
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NOTCH1 3'UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1413
  • Mutation
    Mutation on miR-200c binding site on NOTCH1 3'UTR
  • Entrez Gene
    NOTCH1 (a.k.a. AOS5, AOVD1, TAN1, hN1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Renilla Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer RenLuc3F
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneoRL-Notch1 3'UTR-mir200c-mut was a gift from Yanan Yang (Addgene plasmid # 84596 ; http://n2t.net/addgene:84596 ; RRID:Addgene_84596)

  • For your References section:

    A genetic cell context-dependent role for ZEB1 in lung cancer. Zhang T, Guo L, Creighton CJ, Lu Q, Gibbons DL, Yi ES, Deng B, Molina JR, Sun Z, Yang P, Yang Y. Nat Commun. 2016 Jul 26;7:12231. doi: 10.1038/ncomms12231. 10.1038/ncomms12231 PubMed 27456471
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