FoRC.PGa
(Plasmid
#84584)
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Purpose(Empty Backbone) Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84584 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCasper2
- Backbone size (bp) 7500
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Modifications to backboneTwo double FRT sites were inserted.
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCasper_F
- 3′ sequencing primer pCasper_R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FoRC.PGa was a gift from Francesca Pignoni (Addgene plasmid # 84584 ; http://n2t.net/addgene:84584 ; RRID:Addgene_84584) -
For your References section:
Mutant Analysis by Rescue Gene Excision: New tools for mosaic studies in Drosophila. Zhou Q, Neal SJ, Pignoni F. Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984. 10.1002/dvg.22984 PubMed 27696669
Map uploaded by the depositor.
