pUS232
(Plasmid
#84579)
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Purpose(Empty Backbone) Shuttle and expression vector. Predicted to replicate in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Tetracycline-inducible expression of cloned genes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84579 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUS232
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A derivative of pBBR1MCS-2. Non-mobilisable. Cloning site is compatible with iGEM cloning protocols (EcoRI-NotI-XbaI-SpeI-NotI-PstI) and Golden-Gate cloning protocols (BsaI-BsaI). Blue/white selection is via excision of lacZalpha fragment, rather than interruption of this. Has unique ScaI, BamHI, SmaI, EcoRV, EcoRI, XbaI, StuI, SpeI, PstI, HindIII, ClaI, SalI sites for cloning. Has two NotI and BsaI sites for cloning
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUS232 was a gift from Nicholas Coleman (Addgene plasmid # 84579 ; http://n2t.net/addgene:84579 ; RRID:Addgene_84579)
