pLenti Dest PuroR R1-R2
(Plasmid
#84575)
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Purpose(Empty Backbone) Gateway destination vector expressing Puromycin resistance under a PGK promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMuLE Lenti Dest Neo
- Backbone size (bp) 10359
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Modifications to backboneSubcloned in PuroR using SbfI and MluI
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Vector typeMammalian Expression, Lentiviral ; Gateway
- Promoter PGK
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsDual selection with chloramphenicol and ampicillin required to prevent recombination
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer mPGK_F_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byIan Frew, Addgene #62178
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti Dest PuroR R1-R2 was a gift from Ghassan Mouneimne (Addgene plasmid # 84575 ; http://n2t.net/addgene:84575 ; RRID:Addgene_84575) -
For your References section:
The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Padilla-Rodriguez M, Parker SS, Adams DG, Westerling T, Puleo JI, Watson AW, Hill SM, Noon M, Gaudin R, Aaron J, Tong D, Roe DJ, Knudsen B, Mouneimne G. Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2. 10.1038/s41467-018-05367-2 [pii] PubMed 30061623
Map uploaded by the depositor.
