pLenti Dest BlastR R1-R2
(Plasmid #84574)

• Purpose: (Empty Backbone) Gateway destination vector expressing Blasticidin resistance under a PGK promoter
• Depositing Lab: Ghassan Mouneimne
• Publication: Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2.

Backbone

• Vector backbone: pMuLE Lenti Dest Neo
• Backbone size (bp): 7547
• Modifications to backbone: Subcloned in BlastR using SbfI and MluI
• Vector type: Mammalian Expression, Lentiviral ; Gateway
• Promoter: PGK
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Dual selection with chloramphenicol and ampicillin required to prevent recombination
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: mPGK_F_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ian Frew, Addgene #62178
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry