pET30a-SRAP-delCys2
(Plasmid #84568)

• Purpose: Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2
• Depositing Lab: Douglas Feldman
• Publication: Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi: 10.1016/j.celrep.2017.09.055.

Backbone

• Vector backbone: pET30a
• Backbone manufacturer: Millipore
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6150
• Modifications to backbone: none
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Srap1
• Alt name: Hmces
• Species: M. musculus (mouse)
• Insert Size (bp): 750
• Mutation: deletion of Cys2
• Entrez Gene: Hmces (a.k.a. 8430410A17Rik, C85376)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • S-Tag (N terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry