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Addgene

p3XFlag-YedK
(Plasmid #84567)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84567 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3XFlag-EYFP (Addgene vector 34582).
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YedK
  • Species
    E. coli
  • Insert Size (bp)
    695
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFlag (N terminal on backbone)
    • C-Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer hGH polyA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFlag-YedK was a gift from Douglas Feldman (Addgene plasmid # 84567 ; http://n2t.net/addgene:84567 ; RRID:Addgene_84567)

  • For your References section:

    Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease. Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE. Cell Rep. 2017 Oct 10;21(2):482-494. doi: 10.1016/j.celrep.2017.09.055. 10.1016/j.celrep.2017.09.055 PubMed 29020633
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