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Addgene

pUbC-OsTIR1-myc-IRES-scFv-sfGFP
(Plasmid #84563)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84563 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUbC lentivirus expression vector (2nd generation)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
  • Alt name
    TIR1, IRES, scFv-sfGFP
  • Species
    Synthetic; Oryza Sativa
  • Tag / Fusion Protein
    • myc tag (9x) (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUbC-OsTIR1-myc-IRES-scFv-sfGFP was a gift from Robert Singer (Addgene plasmid # 84563 ; http://n2t.net/addgene:84563 ; RRID:Addgene_84563)
  • For your References section:

    Translation dynamics of single mRNAs in live cells and neurons. Wu B, Eliscovich C, Yoon YJ, Singer RH. Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084. Epub 2016 May 5. 10.1126/science.aaf1084 PubMed 27313041