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pEF-HA-TSAd-d239-74
(Plasmid #84560)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84560 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF HA (pEF BOS derivative)
  • Total vector size (bp) 6868
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TSAd
  • Alt name
    TSAd-d239-74
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1212
  • Mutation
    deletion of aa 239-274
  • Entrez Gene
    SH2D2A (a.k.a. F2771, SCAP, TSAD, VRAP)
  • Promoter EF1a
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-HA-TSAd-d239-74 was a gift from Anne Spurkland (Addgene plasmid # 84560 ; http://n2t.net/addgene:84560 ; RRID:Addgene_84560)

  • For your References section:

    T cell specific adapter protein (TSAd) interacts with Tec kinase ITK to promote CXCL12 induced migration of human and murine T cells. Berge T, Sundvold-Gjerstad V, Granum S, Andersen TC, Holthe GB, Claesson-Welsh L, Andreotti AH, Inngjerdingen M, Spurkland A. PLoS One. 2010 Mar 18;5(3):e9761. doi: 10.1371/journal.pone.0009761. 10.1371/journal.pone.0009761 PubMed 20305788
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