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Addgene

pEF HA-TSAd-CNS
(Plasmid #84558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF HA (pEF BOS derivative)
  • Total vector size (bp) 6753
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TSAd
  • Alt name
    SH2D2A-5
  • Alt name
    C5-9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1150
  • Mutation
    deletion of exon 7
  • Entrez Gene
    SH2D2A (a.k.a. F2771, SCAP, TSAD, VRAP)
  • Promoter EF1a
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE clone 382223

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF HA-TSAd-CNS was a gift from Anne Spurkland (Addgene plasmid # 84558 ; http://n2t.net/addgene:84558 ; RRID:Addgene_84558)

  • For your References section:

    Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7. Granum S, Sundvold-Gjerstad V, Dai KZ, Kolltveit KM, Hildebrand K, Huitfeldt HS, Lea T, Spurkland A. BMC Immunol. 2006 Jul 13;7:15. 10.1186/1471-2172-7-15 PubMed 16839418
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