pNCS-mCyRFP1
(Plasmid #84545)

• Purpose: Bacterial expression of mCyRFP1
• Depositing Lab: Michael Lin
• Publication: Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.

Backbone

• Vector backbone: pNCS
• Backbone size w/o insert (bp): 2834
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCyRFP1
• Insert Size (bp): 702
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • Genbank
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry