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Addgene

gRNA-mCtnnb1
(Plasmid #84542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    CRISPR
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCtnnb1 gRNA
  • Alt name
    gRNA for mouse beta-catenin
  • Species
    Synthetic
  • Insert Size (bp)
    367

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gRNA-mCtnnb1 was a gift from Bon-Kyoung Koo (Addgene plasmid # 84542 ; http://n2t.net/addgene:84542 ; RRID:Addgene_84542)

  • For your References section:

    One-step generation of conditional and reversible gene knockouts. Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156. 10.1038/nmeth.4156 PubMed 28135257
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